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ykuL [2017-10-17 13:46:09]
Molecular weight
16.49 kDa
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
1,485,453 → 1,485,896
The protein
Expression and Regulation
Biological materials
Mutant
MGNA-A773 (ykuL::erm), available at the NBRP B. subtilis, JapanBKE14130 (ykuL::erm trpC2) available in the Bacillus Genetic Stock Center and in Jörg Stülke's lab PubMedGP214 (deletion of ykuJ-ykuK-abbA-darB, replaced by aphA3), available in the Stülke labBKE14130 (ΔdarB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTTBKK14130 (ΔdarB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTTTCGACCCCTTTTC, downstream forward: _UP4_TAGGCTGTACGGTCCTATTT Expression vector
pGP2929 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab FLAG-tag construct
References
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